메뉴 건너뛰기

Corporate Workshop

Home > 프로그램 > Corporate Workshop
Seongcheol HongSeongcheol Hong
Date
6월 30일 11:45-12:15
Speaker
Seongcheol Hong   CV
Affiliation
Thermo Fisher Scientific
Title
Introduction to New products of Thermo Fisher Scientific related to Orbitrap HRMS system for Proteomics
Abstract

Abstract: LCMS analysis using long separation columns and nano-flow rates has been played issential role in both deep-dive discovery proteomics and protein quantitation. General concerns for ‘routine’ application of this approach are reproducibility, robustness, and productivity. To overcome those hurdles, many technical innovations including tandem mass tag (TMT) for protein quantitation and micro-flow for high-throughput analysis were developed. However, lack of quantiation channels and dynamic range of flowmeter were remained as challenges. In this section, new products related to Thermo Orbitrap system, especially Orbitrap Exploris 480, for proteomics will be introduced. Those are Vanquish Neo UHPLC system, FAIMS pro duo, and TMT 18 plex. We believe those products will be helpful for scientists who handle modern techniques in bottom-up proteomics.

 

Sangtae KimSangtae Kim
Date
6월 30일 12:15 - 12:45
Speaker
Sangtae Kim   CV
Affiliation
Bertis Inc
Title
Deep learning and explainable artificial intelligence for targeted proteomics
Abstract

Deep neural networks have led to breakthroughs in discovery proteomics, but their adoption in targeted proteomics has been slow. In clinical proteomics laboratories, researchers spend a significant time on manual peak picking, interference identification, and peak area adjustments to interpret multiple reaction monitoring (MRM) or parallel reaction monitoring (PRM) data. The burden of manual inspection is a major factor limiting transferability, reproducibility, and scalability of targeted proteomics in clinical applications. We present AutoMRM, a targeted proteomics data interpretation tool based on convolutional neural networks (CNN) and explainable artificial intelligence, designed for clinical mass spectrometry laboratories. When applied to MRM and PRM data, AutoMRM shows an accuracy comparable to that of human experts, obviating or significantly reducing the burden of manual inspection.

 

Seung Joon LeeSeung Joon Lee
Date
6월 30일 12:15 - 12:45
Speaker
Seung Joon Lee   CV
Affiliation
Soulbrain Holdings Co., Ltd.
Title
Proteome-wide interrogation of plasma through integration of Proteograph™ technology
Abstract

Deep profiling of highly low abundant proteins such as those present in the plasma proteome on a large scale has been a challenge for traditional methods. Current workflows employ costly, labor-intensive, time-consuming procedures combining depletion of abundant proteins, fractionation, and enrichment of proteins of interest. The Proteograph™ can provide a solution for precise, deep, high-throughput and unbiased global proteomics using a combination of nanoparticles with surface-functionalized physicochemical properties. Here we introduce a new approach to extend the proteome-wide interrogation for plasma proteomes with Proteograph™ providing simultaneous global proteome profiling and analysis of glycosylation, a major part of PTMs, which plays pivotal roles in proper protein functions and is responsible for pathogenesis of various diseases. Leveraging Proteograph™ and optimized LC-MS/MS workflows without any preprocessing, we demonstrate unbiased analysis of glycosylation with global proteome profiling in blood plasma. Compared to controls and enrichment methods for glycopeptide assessment, a number of glycopeptides were exclusively found with more diverse patterning of glycosylation in the Proteograph™. This application can give potential opportunities to provide more effective, rapid and precise profiling of proteomes, facilitating discovery of new actionable disease biomarkers and comprehensive understanding of biological processes.

 

MahoneyMahoney
Date
6월 30일 12:15 - 12:45
Speaker
Mahoney   CV
Affiliation
Seer, Inc
Title
Multi-nanoparticle Workflow Enables Deep Plasma Proteomics at Scale, with Enhanced Precision, and Depths of Coverage.
Abstract

Low abundance proteins including cytokines, chemokines, are difficult to systematically quantify among other proteins in large unbiased plasma proteomics studies. To overcome limitation of deep plasma proteomics in large cohorts, we have developed a fast and scalable technology that employs intricate protein-nano interactions. Introducing a nanoparticle (NP) into a biofluid such as blood plasma leads to the formation of a selective, specific, and reproducible protein corona at the nano-bio interface driven by the relationship between protein-NP affinity, protein abundance and protein-protein interactions. We previously demonstrated that this process, incorporated within the Seer Proteograph™ Product Suite, offers superior performance in terms of depth, breadth, precision, and throughput compared to conventional deep workflows. The ratio of plasma-to-nanoparticles determines the competition between proteins for binding surface, which plays an important role in protein corona composition and can be optimized to enhance and differentiate protein selectivity. Here we investigate effects of different conditions on protein corona composition enabling enhanced performance of Proteograph.

 

Jin Nyoung ChoiJin Nyoung Choi
Date
7월 1일 11:45 - 12:15
Speaker
Jin Nyoung Choi   CV
Affiliation
Agilent Technologies Korea
Title
Streamlined workflow solution with automated sample preparation and advance LC/MS technology for high throughput protein biomarker quantification
Abstract

In translational research, increased throughput as well as increased multiplexing mass spectrometry using MRM-based LC/MS methods have become popular for targeted, bottom-up quantification of protein biomarkers. Researchers often target signature peptides from a limited number of proteins in large cohorts and monitor their expression levels during a specified time period, often leading to hundreds or thousands of biological samples. In such large-volume studies, high-throughput, robustness, and reproducibility are essential when deploying LC/MS methods. For this reason, standard-flow triple quadrupole LC/MS (LC/TQ) is superior to other low-flow LC/MS platforms. In addition to reproducibility and robustness, analytical sensitivity is another important consideration for peptide quantification. The 6495 triple quadrupole LC/MS system coupled with an Agilent Jet Stream (AJS) ionization source is a standard flow-based LC/TQ platform that provides outstanding performance for targeted peptide quantification due to its high sensitivity, reproducibility, and robustness as well as ease of handling and maintenance. Also, routine sample handling tasks common to LC/MS proteomic workflows have been automated using the Agilent AssayMAP Bravo platform and its suite of proteomic tools engineered to bring reproducibility, scalability, protocol portability, and ease-of-use to LC/MS sample preparation.

 

Young Chan KimYoung Chan Kim
Date
7월 1일 11:45 - 12:15
Speaker
Young Chan Kim   CV
Affiliation
MDxK (Molecular Diagnostics Korea Inc.)
Title
Protein Biomarker Discovery to accelerate Research
Abstract

Discovery proteomics research has made significant progress in the past several years; however, the number of protein biomarkers deployed in clinical practice remains rather limited. There are several scientific and procedural gaps between discovery proteomics research and clinical implementation, which have contributed to poor biomarker validity and few clinical applications. The complexity and low throughput of proteomics approaches have added additional barriers for biomarker assay translation to clinical applications. Recently, targeted proteomics have become a powerful tool to bridge the biomarker discovery to clinical validation. In this perspective, we discuss the challenges and strategies in proteomics research from a clinical perspective, and propose several recommendations for discovery proteomics research to accelerate protein biomarker discovery.

Olink’s vision is to accelerate proteomics together with the drug development and research communities to achieve the goal of precision medicine and individualized healthcare, based on a deep understanding of real-time human biology.

The unique technology behind our Olink® Explore platform and Olink® Target 96 and Target 48 panels enables high-throughput, multiplex immunoassays of proteins using minimal volumes of serum, plasma, or almost any other type of biological sample. Here we will show you how our technology delivers this scale of multiplexing and sample throughput without compromising on data quality or assay robustness.

 

Cory ECory E
Date
7월 1일 12:15 -12:45
Speaker
Cory E   CV
Affiliation
Merck KGaA
Title
Racing Through Separations Unhindered: The Use of Monolithic UHPLC Columns for High-Throughput and Robust Analyses
Abstract

Silica monoliths have been employed for high performance liquid chromatography applications since the early 2000's. These columns have specific advantages over traditional, particle packed columns including enhanced matrix tolerance, lower equilibration times, and high flow rate tolerance due to low, overall backpressure. In addition, recent advances in the synthesis of these monoliths have led to monolithic columns being more compatible with mass spectrometry instrumentation in addition to being compatible with large molecule (protein) separations. As separation challenges become more demanding due to the need for high-throughput and/or automation-compatible methods, methods for characterizing complex analytes like monoclonal antibodies and antibody-drug conjugates, and stationary phases that are reliable even with the most demanding, matrix-loaded samples, monoliths are a useful addition to any chromatographer's toolbox.
This presentation will examine a few different case studies where monolithic columns were a unique solution to a difficult separation challenge. After a brief overview of what monoliths are, case studies involving different separation challenges in pharmaceutical and biopharmaceutical environments will be discussed. How the challenge was solved, in addition to method development strategies will be presented. Finally, some unique applications of monolithic UHPLC columns will be presented that showcase the higher degree of flexibility these columns have over more traditional chromatographic columns.

 

브루커 코리아브루커 코리아
Date
7월 1일 12:15 - 12:45
Speaker
브루커 코리아   CV
Affiliation
Bruker Korea Co., LtD
Title
Expanding the horizons of single cell research
Abstract

Mass spectrometric proteomics has become a staple of modern research in understanding biological function and disease mechanisms. Healthy or diseased tissues that seem homogenous are composed of cells with a variety of different proteomes. The challenge of deciphering the proteomes in each single cell – the cell heterogeneity – holds the key to fully understanding its function.

The timsTOF SCP offers a radically improved ion source concept. Combined with parallel accumulation serial fragmentation (PASEF) acquisition methods, it provides extremely high speed and sensitivity to tackle proteomes of single cells or post translational modifications in a few cells that are morphologically or functionally similar.

The timsTOF SCP features a modified ion source geometry that includes 1 mm capillary identification for five times higher ion transfer into an additional higher pressure stage ion funnel and 8-stage differentially pumped vacuum system.

The larger capillary yields ultra-high sensitivity and the additional orthogonal ion reflection and subsequent funnel offers a separate, differentially pumped stage, maintaining the system robustness expected from the timsTOF instrument series. timsControl allows customization of the dia-PASEF window scheme to focus on
the ions of interest. Adjusting the mass isolation width, TIMS range and cycle time allows adaptation of dia-PASEF to different chromatography methods.

Combining low flow liquid chromatography on the Evosep One system with Whisper with the high sensitivity of dia-PASEF on the timsTOF SCP, over 2000 proteins were identified from 500 pg of cell digest and 1500 proteins were identified from 250 pg, demonstrating the sensitivity needed for true single cell proteomics. The proteins identified from 250 pg covered an abundance range of about 4 orders of magnitude, enabling quantitative proteome analysis at single cell level